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Single molecule biophysics lies at the confluence of molecular medicine and nanotechnology. Nanotechnology allows researchers to study the physical processes on which life is based using the simplest model systems -- those that exist on the level of a single molecule or several molecules, but also to unravel fundamental biological processes on the cellular and tissue level. Doing so allows for a better understanding of gene regulation, molecular and cellular signaling, molecular transport in cells and tissue and will lead to improved biosensors and other new technologies.